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Using metabolomics to assess the sub-lethal effects of zinc and boscalid on an estuarine polychaete worm over time.
Sinclair, Georgia M; O'Brien, Allyson L; Keough, Michael; De Souza, David P; Dayalan, Saravanan; Kanojia, Komal; Kouremenos, Konstantinos; Tull, Dedreia L; Coleman, Rhys A; Jones, Oliver A H; Long, Sara M.
Afiliação
  • Sinclair GM; School of BioSciences, The University of Melbourne, Royal Parade, Parkville, VIC, 3052, Australia.
  • O'Brien AL; Centre for Aquatic Pollution Identification and Management (CAPIM), School of BioSciences, The University of Melbourne, Royal Parade, Parkville, VIC, 3052, Australia.
  • Keough M; Aquatic Environmental Stress Research Group, RMIT-University, Plenty Rd, Bundoora, VIC, 3083, Australia.
  • De Souza DP; School of BioSciences, The University of Melbourne, Royal Parade, Parkville, VIC, 3052, Australia.
  • Dayalan S; School of BioSciences, The University of Melbourne, Royal Parade, Parkville, VIC, 3052, Australia.
  • Kanojia K; Metabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, Parkville, VIC, 3010, Australia.
  • Kouremenos K; Metabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, Parkville, VIC, 3010, Australia.
  • Tull DL; CSL Limited, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, Parkville, 3010, Australia.
  • Coleman RA; Metabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, Parkville, VIC, 3010, Australia.
  • Jones OAH; Metabolomics Australia, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, Parkville, VIC, 3010, Australia.
  • Long SM; Trajan Scientific and Medical, 7 Argent Pl, Ringwood, VIC, 3134, Australia.
Metabolomics ; 15(8): 108, 2019 07 31.
Article em En | MEDLINE | ID: mdl-31367897
INTRODUCTION: Zinc is a heavy metal commonly detected in urban estuaries around Australia. Boscalid is a fungicide found in estuaries, both in water and sediment, it enters the system predominantly through agricultural run-off. Zinc is persistent while boscalid breaks down, with a half-life of 108 days. Both contaminants are widely distributed and their effects on ecosystems are not well understood. OBJECTIVES: The aim of this study was to determine the metabolite changes in Simplisetia aequisetis (an estuarine polychaete) following laboratory exposure to a sub-lethal concentration of zinc or boscalid over a 2-week period. METHODS: Individuals were collected at six time points over a 2-week period. Whole polychaete metabolites were extracted and quantified using a multi-platform approach. Polar metabolites were detected using a semi-targeted GC-MS analysis and amine containing compounds were analysed using a targeted LC-MS analysis. Total lipid energy content was also analysed for Simplisetia aequisetis. RESULTS: The pathways that responded to zinc and boscalid exposure were alanine, aspartate and glutamate metabolism (AAG); glycine, serine and threonine metabolism (GST) and metabolites associated with the tricarboxylic acid cycle (TCA). Results showed that changes in total abundance of some metabolites could be detected as early as 24-h exposure. Changes were detected in the metabolites before commonly used total lipid energy assays identified effects. CONCLUSION: A multi-platform approach provided a holistic overview of the metabolomic response to contaminants in polychaetes. This approach shows promise to be used in biomonitoring programs to provide early diagnostic indicators of contamination and exposure.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poliquetos / Compostos de Bifenilo / Cloretos / Niacinamida / Compostos de Zinco / Metabolômica Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Metabolomics Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Austrália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poliquetos / Compostos de Bifenilo / Cloretos / Niacinamida / Compostos de Zinco / Metabolômica Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Metabolomics Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Austrália