Recovery of chicken growth plate by Tanshinoneâ
¡A through wnt/ß-catenin pathway in thiram-induced Tibial Dyschondroplasia.
Ecotoxicol Environ Saf
; 183: 109575, 2019 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-31442808
ABSTRACT
Tibial Dyschondroplasia (TD), a metabolic disease of fast growing poultry birds that effects the growth of bone and cartilage, is characterized by anorexia, mental depression and lameness. Wnt/ß-catenin pathway can mediate the occurrence of TD, and previous study showed the therapeutic effect of Tanshinoneâ
¡A to TD Broilers. However there is no report about the effect of Tanshinoneâ
¡A treating TD broiler chicken through wnt/ß-catenin pathway. The objective of this study was to explore the potential mechanism of how Tanshinone II A treats TD. Hematoxylin and eosin staining was used to study histologic pathology of growth plates. Key gene expressions were tested by western blot and reverse transcription quantitative real-time PCR. Results compared with control groups, showed the TD broilers' growth plate performed significantly better by treating with Tanshinoneâ
¡A. After chickens treated by Tanshinoneâ
¡A, the gene and protein expression of WNT5α and BMP-2 were increased (Pâ¯<â¯0.05), but the ß-catenin were decreased (Pâ¯<â¯0.05), which are all key genes expressed in wnt/ß-catenin pathway. Therefore, Tanshinoneâ
¡A can potentially treat TD by affecting the expression of genes in wnt/ß-catenin pathway and it has availability to use as treatment for TD broilers.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteocondrodisplasias
/
Doenças das Aves Domésticas
/
Abietanos
/
Beta Catenina
/
Via de Sinalização Wnt
/
Lâmina de Crescimento
Limite:
Animals
Idioma:
En
Revista:
Ecotoxicol Environ Saf
Ano de publicação:
2019
Tipo de documento:
Article