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A transplantable tumor model allowing investigation of NY-BR-1-specific T cell responses in HLA-DRB1*0401 transgenic mice.
Das, Krishna; Eisel, David; Vormehr, Mathias; Müller-Decker, Karin; Hommertgen, Adriane; Jäger, Dirk; Zörnig, Inka; Feuerer, Markus; Kopp-Schneider, Annette; Osen, Wolfram; Eichmüller, Stefan B.
Afiliação
  • Das K; Research Group GMP & T Cell Therapy, German Cancer Research Center (DKFZ), Heidelberg, Germany.
  • Eisel D; Division of Virology, Innsbruck Medical University, Innsbruck, Austria.
  • Vormehr M; Faculty of Biosciences, University Heidelberg, Heidelberg, Germany.
  • Müller-Decker K; Research Group GMP & T Cell Therapy, German Cancer Research Center (DKFZ), Heidelberg, Germany.
  • Hommertgen A; Faculty of Biosciences, University Heidelberg, Heidelberg, Germany.
  • Jäger D; Biopharmaceutical New Technologies (BioNTech) Corporation, Mainz, Germany.
  • Zörnig I; Biopharmaceutical New Technologies (BioNTech) Corporation, Mainz, Germany.
  • Feuerer M; University Medical Center of the Johannes Gutenberg University, Mainz, Germany.
  • Kopp-Schneider A; Core Facility Tumor Models, German Cancer Research Center (DKFZ), Heidelberg, Germany.
  • Osen W; Research Group GMP & T Cell Therapy, German Cancer Research Center (DKFZ), Heidelberg, Germany.
  • Eichmüller SB; Department of Molecular & Radiation Oncology, German Cancer Research Center (DKFZ), Heidelberg, Germany.
BMC Cancer ; 19(1): 914, 2019 Sep 13.
Article em En | MEDLINE | ID: mdl-31519152
BACKGROUND: NY-BR-1 has been described as a breast cancer associated differentiation antigen with intrinsic immunogenicity giving rise to endogenous T and B cell responses. The current study presents the first murine tumor model allowing functional investigation of NY-BR-1-specific immune responses in vivo. METHODS: A NY-BR-1 expressing tumor model was established in DR4tg mice based on heterotopic transplantation of stable transfectant clones derived from the murine H2 compatible breast cancer cell line EO771. Composition and phenotype of tumor infiltrating immune cells were analyzed by qPCR and FACS. MHC I binding affinity of candidate CTL epitopes predicted in silico was determined by FACS using the mutant cell line RMA-S. Frequencies of NY-BR-1 specific CTLs among splenocytes of immunized mice were quantified by FACS with an epitope loaded Db-dextramer. Functional CTL activity was determined by IFNγ catch or IFNγ ELISpot assays and statistical analysis was done applying the Mann Whitney test. Tumor protection experiments were performed by immunization of DR4tg mice with replication deficient recombinant adenovirus followed by s.c. challenge with NY-BR-1 expressing breast cancer cells. RESULTS: Our results show spontaneous accumulation of CD8+ T cells and F4/80+ myeloid cells preferentially in NY-BR-1 expressing tumors. Upon NY-BR-1-specific immunization experiments combined with in silico prediction and in vitro binding assays, the first NY-BR-1-specific H2-Db-restricted T cell epitope could be identified. Consequently, flow cytometric analysis with fluorochrome conjugated multimers showed enhanced frequencies of CD8+ T cells specific for the newly identified epitope in spleens of immunized mice. Moreover, immunization with Ad.NY-BR-1 resulted in partial protection against outgrowth of NY-BR-1 expressing tumors and promoted intratumoral accumulation of macrophages. CONCLUSION: This study introduces the first H2-Db-resctricted CD8+ T cell epitope-specific for the human breast cancer associated tumor antigen NY-BR-1. Our novel, partially humanized tumor model enables investigation of the interplay between HLA-DR4-restricted T cell responses and CTLs within their joint attack of NY-BR-1 expressing tumors.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos T / Epitopos de Linfócito T / Cadeias HLA-DRB1 / Antígenos de Neoplasias / Neoplasias Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: BMC Cancer Assunto da revista: NEOPLASIAS Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos T / Epitopos de Linfócito T / Cadeias HLA-DRB1 / Antígenos de Neoplasias / Neoplasias Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: BMC Cancer Assunto da revista: NEOPLASIAS Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Alemanha