Development of a paper-based method to detect Hg2+ in waste water using iturin from Bacillus subtilis.
Appl Microbiol Biotechnol
; 103(20): 8609-8618, 2019 Oct.
Article
em En
| MEDLINE
| ID: mdl-31522284
ABSTRACT
Colorimetric, fluorescence, and paper-based method were developed to measure the Hg2+ level in water using iturin A, a lipopeptide produced by Bacillus subtilis. Firstly, iturin was used to synthesize highly stable and uniformly sized silver nanoparticles (AgNPs). Secondly, the iturin-AgNPs were found to be highly selective and sensitive to Hg2+. The absorbance of the reaction system showed a good linear correlation with the Hg2+ concentration from 0.5 to 5 mg/L at 450 nm in the UV-Vis spectroscopy detection with the limit of detection (LOD) of 0.5 mg/L. When the reaction system was detected by fluorescence measurement, a good linear relationship was found between the fluorescence intensity and Hg2+ concentration from 0.05 to 0.5 mg/ at 415 nm with the LOD of 0.05 mg/L. Lastly, a paper-based detection method was developed. The developed method was successfully used to detect Hg2+ in contaminated polluted waters and showed acceptable results in terms of sensitivity, selectivity and stability. The paper-based method could distinguish Hg2+ at levels higher than 0.05 mg/L, thereby meeting the guidelines of the effluent quality standard for industries (0.05 mg/L). In summary, this method can be used daily by various industries to monitor the Hg2+ level in effluent water.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos Cíclicos
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Poluentes Químicos da Água
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Técnicas de Química Analítica
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Colorimetria
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Águas Residuárias
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Fluorometria
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Mercúrio
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China