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The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue.
Voigt, Fabian F; Kirschenbaum, Daniel; Platonova, Evgenia; Pagès, Stéphane; Campbell, Robert A A; Kastli, Rahel; Schaettin, Martina; Egolf, Ladan; van der Bourg, Alexander; Bethge, Philipp; Haenraets, Karen; Frézel, Noémie; Topilko, Thomas; Perin, Paola; Hillier, Daniel; Hildebrand, Sven; Schueth, Anna; Roebroeck, Alard; Roska, Botond; Stoeckli, Esther T; Pizzala, Roberto; Renier, Nicolas; Zeilhofer, Hanns Ulrich; Karayannis, Theofanis; Ziegler, Urs; Batti, Laura; Holtmaat, Anthony; Lüscher, Christian; Aguzzi, Adriano; Helmchen, Fritjof.
Afiliação
  • Voigt FF; Brain Research Institute, University of Zurich, Zurich, Switzerland. voigt@hifo.uzh.ch.
  • Kirschenbaum D; Neuroscience Center Zurich, University of Zurich, ETH Zurich, Zurich, Switzerland. voigt@hifo.uzh.ch.
  • Platonova E; University Hospital Zurich, Zurich, Switzerland.
  • Pagès S; Center for Microscopy and Image Analysis, University of Zurich, Zurich, Switzerland.
  • Campbell RAA; Wyss Center for Bio- and Neuroengineering, Geneva, Switzerland.
  • Kastli R; Department of Basic Neurosciences, Geneva Neuroscience Center, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
  • Schaettin M; Sainsbury Wellcome Centre for Neural Circuits and Behaviour, London, UK.
  • Egolf L; Brain Research Institute, University of Zurich, Zurich, Switzerland.
  • van der Bourg A; Neuroscience Center Zurich, University of Zurich, ETH Zurich, Zurich, Switzerland.
  • Bethge P; Neuroscience Center Zurich, University of Zurich, ETH Zurich, Zurich, Switzerland.
  • Haenraets K; Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.
  • Frézel N; Brain Research Institute, University of Zurich, Zurich, Switzerland.
  • Topilko T; Neuroscience Center Zurich, University of Zurich, ETH Zurich, Zurich, Switzerland.
  • Perin P; Brain Research Institute, University of Zurich, Zurich, Switzerland.
  • Hillier D; Neuroscience Center Zurich, University of Zurich, ETH Zurich, Zurich, Switzerland.
  • Hildebrand S; Brain Research Institute, University of Zurich, Zurich, Switzerland.
  • Schueth A; Neuroscience Center Zurich, University of Zurich, ETH Zurich, Zurich, Switzerland.
  • Roebroeck A; Neuroscience Center Zurich, University of Zurich, ETH Zurich, Zurich, Switzerland.
  • Roska B; Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
  • Stoeckli ET; Neuroscience Center Zurich, University of Zurich, ETH Zurich, Zurich, Switzerland.
  • Pizzala R; Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
  • Renier N; ICM-Brain & Spine Institute, Paris, France.
  • Zeilhofer HU; Department of Brain and Behavioural Sciences, University of Pavia, Pavia, Italy.
  • Karayannis T; Hungarian Academy of Sciences Research Centre for Natural Sciences, Budapest, Hungary.
  • Ziegler U; Faculty of Information Technology and Bionics, Pazmany Peter Catholic University, Budapest, Hungary.
  • Batti L; Faculty of Psychology & Neuroscience, Maastricht University, Maastricht, Netherlands.
  • Holtmaat A; Faculty of Psychology & Neuroscience, Maastricht University, Maastricht, Netherlands.
  • Lüscher C; Faculty of Psychology & Neuroscience, Maastricht University, Maastricht, Netherlands.
  • Aguzzi A; Friedrich Miescher Institute Basel, Basel, Switzerland.
  • Helmchen F; Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
Nat Methods ; 16(11): 1105-1108, 2019 11.
Article em En | MEDLINE | ID: mdl-31527839
ABSTRACT
Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ( www.mesospim.org ).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microscopia de Fluorescência Limite: Animals Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Suíça
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microscopia de Fluorescência Limite: Animals Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Suíça