Endothelialization of Titanium Surfaces by Bioinspired Cell Adhesion Peptide Coatings.
Bioconjug Chem
; 30(10): 2664-2674, 2019 10 16.
Article
em En
| MEDLINE
| ID: mdl-31532989
ABSTRACT
Common interventional therapies for cardiovascular occlusive diseases, such as the implantation of stents, are at risk of complications like thrombosis or restenosis. Drug-eluting stents have improved patency but simultaneously worsen the endothelialization of the implant. Here, we present a novel peptide coating derived from three proteins of the extracellular matrix named fibronectin, laminin, and elastin. Their active sequences RGD, SIKVAV, and VGVAPG were immobilized onto titanium surfaces by a carrier peptide containing l-3,4-dihydroxyphenylalanine (DOPA). Simultaneous functionalization of the carrier peptide with cyclic c[RGDfK] and SIKVAV had the most potent influence on adhesion, proliferation, viability, and angiogenesis of endothelial cells. By presentation of two adhesion peptides in one molecule, a synergistic enhancement of cell-surface interactions was achieved. Overall, this work clearly demonstrates the advantages of spatially defined peptide coatings for the endothelialization of titanium and thus describes a promising approach for the coating of stents.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
/
Titânio
/
Adesão Celular
/
Células Endoteliais da Veia Umbilical Humana
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Bioconjug Chem
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Alemanha