Functional significance of MAIT cells in psoriatic arthritis.
Cytokine
; 125: 154855, 2020 01.
Article
em En
| MEDLINE
| ID: mdl-31541902
ABSTRACT
BACKGROUND:
Mucosal-associated invariant T (MAIT) cells are gaining more relevance for autoimmune diseases because of its (i) innate and adaptive immune response (ii) tissue homing properties (iii) production of IL-17A. These cells are predominantly CD8+ cells, because of its strong association with MHC-I. Tc17 CD8+/MAIT cells likely to have a critical role in psoriatic arthritis (PsA). Herein, we have explored pathological significance of MAIT cell in PsA.METHODS:
Peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cells (SFMC) were collected from age/sex matched (nâ¯=â¯10 for each) PsA, rheumatoid arthritis (RA) and osteoarthritis patients (OA). Hi-D FACS studies were performed (i) activated memory cells (CD3+CD45RO+) T cells were identified (ii) gating strategies were made to identity the MAIT (CD3+Vα7.2TCR+CD161hi) cells, its phenotype pattern; and functional significance in respect to IL-17A production and responsiveness to human rIL-23. Anti CD3/CD28 ab cocktail was used to activate cells along with rIL-23 to culture and enrich the MAIT cells. The percentages of each cell population and the mean fluorescence intensity (MFI) were analyzed using Flow Jo software.RESULTS:
MAIT cells were enriched in synovial fluid of PsA (4.29⯱â¯0.82%) compared to PBMC (1.04⯱â¯0.71). With stimulation, SFMC MAIT cells produced significantly more IL-17A (32.66⯱â¯4.01%) compared to that of RA (23.93⯱â¯2.81%, pâ¯<â¯0.05) and OA (5.02⯱â¯0.16%, pâ¯<â¯0.05). MAIT cells were predominantly CD8+ (>80%). Significant upregulation of IL-23R was noted in synovial fluid MAIT cells of PsA (24.97⯱â¯2.33%, pâ¯<â¯0.001) and RA (21.93⯱â¯2.29%, pâ¯<â¯0.001) compared to that of OA (2.13⯱â¯2.29). This IL-23R was functionally active as evidenced by profound mitotic effect in presence of rIL-23.CONCLUSION:
MAIT cells are poly functional; produce multiple cytokines (IL-17A, IFN-γ, TNF-α). Here, we demonstrated synovial fluid MAIT cells as a major source of IL-17A and majority of MAIT cells were CD8+. Functionally active IL-23R on these migrated MAIT cells brings a new dimension. They may not need MR1 associated activation rather lesional IL-23 in the synovium can independently regulate these critical Tc17 CD8+ MAIT cells. Thus, these cells likely to be a part of the IL-23/IL-17A cytokine network and play a critical role in the pathogenesis of PsA.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Artrite Psoriásica
/
Receptores de Interleucina
/
Interleucina-17
/
Células T Invariantes Associadas à Mucosa
Limite:
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Cytokine
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos