Ability of real-time PCR for differential diagnosis of various forms of cutaneous leishmaniasis: a comparative study with histopathology.
BMC Res Notes
; 12(1): 615, 2019 Sep 23.
Article
em En
| MEDLINE
| ID: mdl-31547842
ABSTRACT
OBJECTIVE:
Histopathological studies suggest that parasite load is different between acute and chronic forms of cutaneous leishmaniasis (CL). However, highly sensitive detection methods are still needed to distinguish different forms of leishmaniasis. In the present study, we developed a quantitative real-time polymerase chain reaction (PCR) to detect and quantify Leishmania tropica parasites in paraffin-embedded tissue samples.RESULTS:
The ability of real-time PCR for leishmania detection was higher than histopathological evaluation. The quantitative real-time PCR (qPCR) quantified parasite loads were highly correlated with microscopic results (r = 0.598; P < 0.001). Among patients, the parasite load was inversely correlated with disease duration (acute CL lesions had very higher parasite load than chronic CL lesions), but there was no difference in the parasite load according to the patients' age and sex as well as location of the lesions. In contrast to Ridley scoring system (P < 0.001), there were no statistically significant differences in the relative number of parasites among the lupoid and non-lupoid forms of chronic lesions in real-time PCR (P = 0.549), which indicates the superiority of histopathological evaluation for chronic forms differentiation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Leishmania tropica
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DNA de Protozoário
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Leishmaniose Cutânea
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
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Adult
/
Aged
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Child
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
BMC Res Notes
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Irã