The association between collagen and bone biomarkers and radiographic osteoarthritis in the distal tarsal joints of horses.
Equine Vet J
; 52(3): 391-398, 2020 May.
Article
em En
| MEDLINE
| ID: mdl-31596508
ABSTRACT
BACKGROUND:
Osteoarthritis (OA) of the distal intertarsal (DIT) and tarsometatarsal (TMT) joints occurs commonly. Synovial fluid (SF) biomarkers of collagen and bone turnover have potential clinical value.OBJECTIVES:
To measure SF biomarker concentrations from DIT and TMT joints in adult horses and determine if they correlate with radiographic OA severity and are higher in joints with radiographic OA compared to controls. STUDYDESIGN:
Cross-sectional.METHODS:
Radiographic OA of DIT and TMT joints was evaluated from adult horses (5-35 years old). Overall radiographic scores divided horses into those with mild or moderate radiographic OA (16 joints from 9 horses) or controls (13 joints from 9 horses). Direct biomarkers of OA (Carboxypropeptide of type II collagen = CPII, carboxy-neoepitope of type II collagen exposed after collagenase-cleavage = C2C, Bone alkaline phosphatase = BAP and Chondroitin sulfate epitope = CS846) were measured via ELISA and CPII/C2C was calculated. Biomarkers were correlated with radiographic findings and concentrations from those with radiographic OA to control joints and were compared.RESULTS:
Concentrations of CPII (R = 0.84, P<0.001), C2C (R = 0.69, P<0.001) and BAP (R = 0.41, P = 0.03) as well as CPII/C2C (R = 0.69, P<0.001) values positively correlated with overall radiographic scores. Adjusted means ± s.d., after controlling for age, for CPII (P<0.001), C2C (P<0.001), CPII/C2C (P = 0.004) and BAP (P = 0.05) were significantly higher in DIT and TMT joints with radiographic OA (CPII 2174.45 ± 1064.01; C2C 233.52 ± 51.187; CPII/C2C 9.01 ± 4.09; BAP 21.98 ± 15.34) compared to controls (CPII 594.53 ± 463.05; C2C 153.12 ± 48.95; CPII/C2C 3.96 ± 2.38; BAP 12.76 ± 3.61). CPII (P<0.001), C2C (P = 0.001) and CPII/C2C (P = 0.001) were significantly higher with moderate radiographic OA (CPII 2444.61 ± 772.78; C2C 248.90 ± 44.94; CPII/C2C 9.47 ± 2.97) compared to controls (CPII 658.38 ± 417.36; C2C 156.49 ± 47.61; CPII/C2C 4.15 ± 2.04), with CPII also showing significantly higher concentrations (P = 0.04) with mild radiographic OA compared (1515.00 ± 584.95) to controls (658.38 ± 417.36). There were no differences in CS846 concentrations between radiographic OA and control joints. Age positively correlated with CPII (R = 0.48, P = 0.01) and C2C (R = 0.44, P = 0.02) concentrations. MAINLIMITATIONS:
Radiographic OA was assessed, not clinical lameness. Controls were not age-matched to those with spontaneous radiographic OA.CONCLUSIONS:
There is an association between collagen (CPII, C2C and CPII/C2C) and bone (BAP) biomarkers and radiographic OA in the distal tarsal joints of horses.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoartrite
/
Articulações Tarsianas
/
Doenças dos Cavalos
Tipo de estudo:
Observational_studies
/
Prevalence_studies
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Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Equine Vet J
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos