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Identification and characterization of primordial germ cells in a vocal learning Neoaves species, the zebra finch.
Jung, Kyung Min; Kim, Young Min; Keyte, Anna L; Biegler, Matthew T; Rengaraj, Deivendran; Lee, Hong Jo; Mello, Claudio V; Velho, Tarciso A F; Fedrigo, Olivier; Haase, Bettina; Jarvis, Erich D; Han, Jae Yong.
Afiliação
  • Jung KM; Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, South Korea.
  • Kim YM; Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, South Korea.
  • Keyte AL; Laboratory of Neurogenetics of Language, The Rockefeller University, New York, New York, USA.
  • Biegler MT; Laboratory of Neurogenetics of Language, The Rockefeller University, New York, New York, USA.
  • Rengaraj D; Department of Neurobiology, Duke University Medical Center, Durham, North Carolina, USA.
  • Lee HJ; Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, South Korea.
  • Mello CV; Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, South Korea.
  • Velho TAF; Department Behavioral Neuroscience, Oregon Health and Science University, Portland, Oregon, USA.
  • Fedrigo O; The Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
  • Haase B; Brain Institute, Federal University of Rio Grande do Norte, Natal, Brazil.
  • Jarvis ED; Laboratory of Vertebrate Genomes, The Rockefeller University, New York, New York, USA.
  • Han JY; Laboratory of Vertebrate Genomes, The Rockefeller University, New York, New York, USA.
FASEB J ; 33(12): 13825-13836, 2019 12.
Article em En | MEDLINE | ID: mdl-31604057
The zebra finch has been used as a valuable vocal learning animal model for human spoken language. It is representative of vocal learning songbirds specifically, which comprise half of all bird species, and of Neoaves broadly, which comprise 95% of all bird species. Although transgenesis in the zebra finch has been accomplished, it is with a very low efficiency of germ-line transmission and far from the efficiency with a more genetically tractable but vocal nonlearning species, the chicken (a Galloanseriformes). To improve germ-line transmission in the zebra finch, we identified and characterized its primordial germ cells (PGCs) and compared them with chicken. We found striking differences between the 2 species, including that zebra finch PGCs were more numerous, more widely distributed in early embryos before colonization into the gonads, had slower timing of colonization, and had a different developmental gene-expression program. We improved conditions for isolating and culturing zebra finch PGCs in vitro and were able to transfect them with gene-expression vectors and incorporate them into the gonads of host embryos. Our findings demonstrate important differences in the PGCs of the zebra finch and advance the first stage of creating PGC-mediated germ-line transgenics of a vocal learning species.-Jung, K. M., Kim, Y. M., Keyte, A. L., Biegler, M. T., Rengaraj, D., Lee, H. J., Mello, C. V., Velho, T. A. F., Fedrigo, O., Haase, B., Jarvis, E. D., Han, J. Y. Identification and characterization of primordial germ cells in a vocal learning Neoaves species, the zebra finch.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tentilhões / Células Germinativas / Aprendizagem Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: FASEB J Assunto da revista: BIOLOGIA / FISIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Coréia do Sul

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tentilhões / Células Germinativas / Aprendizagem Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: FASEB J Assunto da revista: BIOLOGIA / FISIOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Coréia do Sul