A flow cytometric granularity assay for the quantification of infectious virus.
Vaccine
; 37(47): 7090-7099, 2019 11 08.
Article
em En
| MEDLINE
| ID: mdl-31630940
ABSTRACT
A flow cytometry-based assay was developed to assess the infective titer of two recombinant viruses a recombinant herpes simplex type 2 (rHSV-2) and a recombinant canary pox (rALVAC.gfp). This method uses granularity of infected Vero and QT-35 cells, respectively, and correlates this to the infectious titer of virus samples. The percent of the cell populations with a high level of granularity could accurately be correlated to viral titers obtained through a traditional plaque assay, with R2 values greater than 0.8 using a semi-logarithmic scale. This approach offers a rapid, high-throughput method for infectious virus titration with similar accuracy to a traditional plaque assay.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Viroses
/
Herpesvirus Humano 2
/
Citometria de Fluxo
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Canadá