Curcumin effects on cell proliferation, angiogenesis and metastasis in colorectal cancer.

ABSTRACT

PURPOSE: Curcumin is a natural phytopolyphenol compound isolated from the root of turmeric (Curcuma longa) and possesses a wide range of biological properties. The purpose of this study was to evaluate the antiproliferative, wound healing, anti-invasive and anti-migrative effects of curcumin on HCT-116 and LoVo colorectal cancer cell lines. METHODS: The antiproliferative activity of 2.5-75 µM curcumin was tested on HCT-116 and LoVo colorectal cell lines and the viability of the cells was tested with WST-1 reagent by using ELISA plate reader at 450 nm. xCELLigence RTCA DP system was used for the detection of anti-invasive and anti-migrative effects of curcumin. RESULTS: The IC50 of curcumin was 10±0.03 for HCT-116 and 20±0.05 µM for LoVo cell lines. The IC50 of curcumin (10µM for HCT-116 and 20 µM for LoVo) showed anti-metastatic activity on these cell lines. CONCLUSION: This study showed that curcumin could be evaluated as a promising anti-cancer agent for human colorectal cancer.