Ultrabright fluorescent silica nanoparticles for in vivo targeting of xenografted human tumors and cancer cells in zebrafish.
Nanoscale
; 11(46): 22316-22327, 2019 Nov 28.
Article
em En
| MEDLINE
| ID: mdl-31724677
ABSTRACT
New ultrabright fluorescent silica nanoparticles capable of the fast targeting of epithelial tumors in vivo are presented. The as-synthesized folate-functionalized ultrabright particles of 30-40 nm are 230 times brighter than quantum dots (QD450) and 50% brighter than the polymer dots with similar spectra (excitation 365 nm and emission 486 nm). To decrease non-specific targeting, particles are coated with polyethylene glycol (PEG). We demonstrate the in vivo targeting of xenographic human cervical epithelial tumors (HeLa cells) using zebrafish as a model system. The particles target tumors (and probably even individual HeLa cells) as small as 10-20 microns within 20-30 minutes after blood injection. To demonstrate the advantages of ultrabrightness, we repeated the experiments with similar but 200× less bright particles. Compared to those, ultrabright particles showed â¼3× faster tumor detection and â¼2× higher relative fluorescent contrast of tumors/cancer cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dióxido de Silício
/
Nanopartículas
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Neoplasias
Limite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Nanoscale
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Estados Unidos