Photoelectrochemical competitive immunosensor for 17ß-estradiol detection based on ZnIn2S4@NH2-MIL-125(Ti) amplified by PDA NS/Mn:ZnCdS.
Biosens Bioelectron
; 148: 111739, 2020 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-31731075
ABSTRACT
A competitive-type PEC immunosensor for 17ß-estradiol (E2) detection was successfully fabricated using ZnIn2S4@NH2-MIL-125(Ti) composite as matrix. The excellent PEC behavior of ZnIn2S4@NH2-MIL-125(Ti) composite could be attributed to that the Ti4+-Ti3+ intervalence cycles in the titanium oxo-cluster of NH2-MIL-125(Ti) as well as the matching energy level between ZnIn2S4 and NH2-MIL-125(Ti) promote the migration and separation of photocarrier. Besides, polydopamine (PDA) with abundant amino- and quinone-groups was selected to further improve the PEC signals and capture antibody, which implement through the covalent bonding of PDA and BSA-E2 or carboxyl-group functionalized MnZnCdS QDs in the competitive-type strategy. Concretely, the quinone functional groups in PDA film was applied to immobilize BSA-E2 through Michael reactions, and the PDA nanosphere loaded MnZnCdS quantum dot (PDA NS/MnZnCdS QDs) was used as antibodies' labels to amplify PEC signals. After PDA NS/MnZnCdS-anti-E2 immobilized on the modified electrode, a remarkable increase of photocurrent signal was observed owing to the specific bonding of antigen and antibody. Based on the competitive binding of PDA NS/MnZnCdS-anti-E2 with either free E2 or bovine serum albumin (BSA)-E2 causing the change of the photocurrent signal, the standard sample free E2 could be accuracy detect. Under optimal conditions, the competitive-type PEC immunosensor exhibited the linear range from 0.0005â¯ng/mL to 20â¯ng/mL and a limit detection of 0.3â¯pg/mL (S/Nâ¯=â¯3). Meanwhile, the acceptable stability, selectivity and reproducibility of the proposed PEC immunosensing platform indicating the promising detection of small molecular environmental pollutants.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polímeros
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Titânio
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Poluentes Químicos da Água
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Técnicas Biossensoriais
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Estradiol
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Nanocompostos
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Indóis
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biosens Bioelectron
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article