Hypertonic saccharide solution delays pyroptosis in murine macrophages regardless of the membrane binding of gasdermin D N-terminal.
Eur J Immunol
; 50(3): 464-467, 2020 03.
Article
em En
| MEDLINE
| ID: mdl-31756255
ABSTRACT
Pyroptosis is a type of programmed lytic cell death that could be activated by either the canonical or noncanonical inflammasome pathway. In this study, we aimed to examine the effect of hypertonic solution on noncanonical pyroptosis in macrophage. We found that although hypertonic solution had a general inhibitory effect on noncanonical pyroptosis, the underlying mechanism varied by the solute causing hypertonicity. Specifically, hypertonic NaCl or KCl solution inhibited the cleavage of gasdermin D, the pore-forming protein in pyroptosis, whereas hypertonic saccharide solution did not affect the cleavage or membrane binding of gasdermin D. In this case, nevertheless, pyroptosis was still inhibited as evidenced by the preserved mitochondria activity and cell membrane permeability.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Ligação a Fosfato
/
Peptídeos e Proteínas de Sinalização Intracelular
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Piroptose
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Soluções Hipertônicas
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Macrófagos
Limite:
Animals
Idioma:
En
Revista:
Eur J Immunol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China