Chemoenzymatic Semisynthesis of Proteins.
Chem Rev
; 120(6): 3051-3126, 2020 03 25.
Article
em En
| MEDLINE
| ID: mdl-31774265
ABSTRACT
Protein semisynthesis-defined herein as the assembly of a protein from a combination of synthetic and recombinant fragments-is a burgeoning field of chemical biology that has impacted many areas in the life sciences. In this review, we provide a comprehensive survey of this area. We begin by discussing the various chemical and enzymatic methods now available for the manufacture of custom proteins containing noncoded elements. This section begins with a discussion of methods that are more chemical in origin and ends with those that employ biocatalysts. We also illustrate the commonalities that exist between these seemingly disparate methods and show how this is allowing for the development of integrated chemoenzymatic methods. This methodology discussion provides the technical foundation for the second part of the review where we cover the great many biological problems that have now been addressed using these tools. Finally, we end the piece with a short discussion on the frontiers of the field and the opportunities available for the future.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Enzimas
Limite:
Humans
Idioma:
En
Revista:
Chem Rev
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos