Extraction, characterization and antioxidant activity of polysaccharide from Pouteria campechiana seed.
Carbohydr Polym
; 229: 115409, 2020 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-31826479
ABSTRACT
In this study, the seed polysaccharides (PCSP) was ultrasonic-assisted extracted from Pouteria campechiana and optimized by response surface method (RSM). After separation and purification by DEAE-52 cellulose column and Sephadex G-75 glucan gel column, the pure polysaccharide component of PCSPa-1 was obtained, and its structure and antioxidant activity were analyzed. The results showed that the optimal parameters of PCSP with maximum yields (15.94%) were ultrasonic temperature of 79⯰C, ultrasonic time of 69â¯min, and liquid to material ratio of 41â¯mL/g. The molecular weight of PCSPa-1 was 67900â¯Da. PCSPa-1 consisted of glucose and mannose with a molar ratio of 86.654.62, and the glycosidic bond mainly included â4)-α-d-Glc(1â and â6)-α-d-Glc(1â. Scanning electron microscopy showed that PCSPa-1 was a strip structure with a smooth surface. In addition, PCSPa-1 had strong scavenging capacity to 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis- (3-ethylbenzthiaz oline-6-sulphonic acid) (ABTS), hydroxyl radical, and superoxide radical. Polysaccharides of Pouteria campechiana seeds could be exploited as a natural antioxidant.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Pouteria
/
Antioxidantes
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China