In vitro antioxidant and enzyme inhibitory properties of the n-butanol fraction of Senna podocarpa (Guill. and Perr.) leaf.
J Basic Clin Physiol Pharmacol
; 31(1)2019 Dec 25.
Article
em En
| MEDLINE
| ID: mdl-31874099
ABSTRACT
Background This study evaluates the antioxidant activity and enzyme inhibitory properties of the n-butanol fraction of Senna podocarpa leaves on α-amylase, α-glucosidase, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, arginase, phosphodiesterase 5 (PDE-5), and angiotensin converting enzyme (ACE). Methods The total phenol and flavonoids, iron (Fe) chelation, and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenging were used to determine the antioxidant activity, and the inhibitory activities of α-glucosidase, α-amylase, AChE, BChE, tyrosinase, arginase, PDE-5 and ACE were also assessed. Results The n-butanol fraction of S. podocarpa shows high total phenol and total flavonoid contents. The n-butanol fraction of S. podocarpa leaves also chelates Fe2+ and ABTS radicals. The n-butanol fraction of S. podocarpa leaves also inhibited α-glucosidase, α-amylase, AChE, BChE, tyrosinase, arginase, PDE-5, and ACE at the concentration tested. Chromatographic analysis displayed the presence of ß-elemene, phytol and caryophyllene oxide chrysophanol, 3-oxo-methyl ester, α-humulene, ß-caryophyllene, rhein, emodin, and α-copaene. Conclusions Hence, the n-butanol fraction of S. podocarpa leaves demonstrates encouraging feat in controlling and/or managing cognitive dysfunction such as Alzheimer's disease and also hypertension, diabetes, erectile dysfunction, endothelial dysfunction, and hyperpigmentation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Folhas de Planta
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1-Butanol
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Inibidores Enzimáticos
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Fabaceae
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Antioxidantes
Limite:
Animals
Idioma:
En
Revista:
J Basic Clin Physiol Pharmacol
Assunto da revista:
FARMACOLOGIA
/
FISIOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article