Widespread activation of developmental gene expression characterized by PRC1-dependent chromatin looping.
Sci Adv
; 6(2): eaax4001, 2020 01.
Article
em En
| MEDLINE
| ID: mdl-31950077
ABSTRACT
Polycomb repressive complexes 1 and 2 have been historically described as transcriptional repressors, but recent reports suggest that PRC1 might also support activation, although the underlying mechanisms remain elusive. Here, we show that stage-specific PRC1 binding at a subset of active promoters and enhancers during Drosophila development coincides with the formation of three-dimensional (3D) loops, an increase in expression during development and repression in PRC1 mutants. Dissection of the dachshund locus indicates that PRC1-anchored loops are versatile architectural platforms that persist when surrounding genes are transcriptionally active and fine-tune their expression. The analysis of RING1B binding profiles and 3D contacts during neural differentiation in mice suggests that this role is conserved in mammals.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Regulação da Expressão Gênica no Desenvolvimento
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Drosophila melanogaster
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Genes Controladores do Desenvolvimento
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Complexo Repressor Polycomb 1
Limite:
Animals
Idioma:
En
Revista:
Sci Adv
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
França