Genotyping and drug-resistance epidemiology of mycobacterium tuberculosis in Xuzhou, China.
Int J Clin Exp Pathol
; 10(9): 9675-9682, 2017.
Article
em En
| MEDLINE
| ID: mdl-31966848
ABSTRACT
BACKGROUND:
To explore the genetic diversity and drug resistance status of MTB in Xuzhou, China.METHODS:
A total of 325 clinical MTB strains were genotyped by spacer-oligonucleotide typing (spoligotyping) and mycobacterial interspersed repetitive unit variable number of tandem repeats (MIRU-VNTR). Phenotypic resistance was assessed by drug susceptibility testing (DST).RESULT:
Based on the spoligotyping method, 325 MTB isolates were classified into 5 known genotypes and 12 unknown genotypes, and the largest branch comprised 268 strains belonging to the Beijing family. Based on the 15-loci VNTR typing method, 325 MTB isolates were divided into 35 clusters and 220 unique patterns. Compared to the low discriminatory power of spoligotyping genotyping (HGDI = 0.3444), 15-loci VNTR genotyping had a significantly higher discriminatory power for all strains (HGDI = 0.9980), particularly for the Beijing family strains (HGDI = 0.9892). When spoligotyping and 15-loci VNTR methods were used together, the discriminatory power increased to 0.9991. The Beijing family strain presented increased risks for developing multi-drug resistance TB (P < 0.05).CONCLUSION:
The Beijing family isolates is the most prevalent strains in Xuzhou. Spoligotyping, in combination with 15-loci MIRU-VNTR, is useful for epidemiological analysis of MTB transmission in Xuzhou.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Screening_studies
Idioma:
En
Revista:
Int J Clin Exp Pathol
Assunto da revista:
PATOLOGIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China