Conformation-specific inhibitors of activated Ras GTPases reveal limited Ras dependency of patient-derived cancer organoids.

Wiechmann, Svenja; Maisonneuve, Pierre; Grebbin, Britta M; Hoffmeister, Meike; Kaulich, Manuel; Clevers, Hans; Rajalingam, Krishnaraj; Kurinov, Igor; Farin, Henner F; Sicheri, Frank; Ernst, Andreas

Wiechmann, Svenja; Maisonneuve, Pierre; Grebbin, Britta M; Hoffmeister, Meike; Kaulich, Manuel; Clevers, Hans; Rajalingam, Krishnaraj; Kurinov, Igor; Farin, Henner F; Sicheri, Frank; Ernst, Andreas.

Afiliação

Wiechmann S; Institute of Biochemistry II, Goethe University Frankfurt-Medical Faculty, University Hospital, 60596 Frankfurt am Main, Germany.
Maisonneuve P; Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Project Group Translational Medicine and Pharmacology TMP, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany.
Grebbin BM; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario M5G 1X5, Canada.
Hoffmeister M; German Cancer Consortium (DKTK), 69120 Heidelberg, Germany.
Kaulich M; Georg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy, 60596 Frankfurt am Main, Germany.
Clevers H; German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
Rajalingam K; Institute of Biochemistry II, Goethe University Frankfurt-Medical Faculty, University Hospital, 60596 Frankfurt am Main, Germany.
Kurinov I; Institute of Biochemistry, Brandenburg Medical School (MHB) Theodor Fontane, 14770 Brandenburg an der Havel, Germany.
Farin HF; Institute of Biochemistry II, Goethe University Frankfurt-Medical Faculty, University Hospital, 60596 Frankfurt am Main, Germany.
Sicheri F; Frankfurt Cancer Institute, 60596 Frankfurt am Main, Germany.
Ernst A; Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands.

J Biol Chem ; 295(14): 4526-4540, 2020 04 03.

Article em En | MEDLINE | ID: mdl-32086379

Assuntos

Proteínas Proto-Oncogênicas c-raf/metabolismo; Proteínas ras/metabolismo; Apoptose; Sítios de Ligação; Linhagem Celular Tumoral; Neoplasias Colorretais/metabolismo; Neoplasias Colorretais/patologia; MAP Quinases Reguladas por Sinal Extracelular/metabolismo; Humanos; Simulação de Dinâmica Molecular; Mutagênese; Fosfatidilinositol 3-Quinases/metabolismo; Ligação Proteica; Domínios Proteicos; Estrutura Terciária de Proteína; Proto-Oncogene Mas; Proteínas Proto-Oncogênicas c-raf/química; Proteínas Proto-Oncogênicas c-raf/genética; Proteínas Recombinantes/biossíntese; Proteínas Recombinantes/química; Proteínas Recombinantes/isolamento & purificação; Transdução de Sinais; Proteínas ras/antagonistas & inibidores; Proteínas ras/genética

Palavras-chave

CRAF RBD; GTPase; MAPK pathway; Ras signaling; Ras-binding domain; cancer; cancer organoids; cell signaling; colorectal cancer; conformation-specific inhibitors; crystal structure; oncogenic Ras; organoids; phage display; protein design; protein engineering; protein-protein interaction; substrate specificity

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas ras / Proteínas Proto-Oncogênicas c-raf Limite: Humans Idioma: En Revista: J Biol Chem Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Alemanha

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google