Heme peroxidase-Trapping intermediates by cryo neutron crystallography.

Kwon, Hanna; Schrader, Tobias E; Ostermann, Andreas; Blakeley, Matthew P; Raven, Emma L; Moody, Peter C E

Kwon, Hanna; Schrader, Tobias E; Ostermann, Andreas; Blakeley, Matthew P; Raven, Emma L; Moody, Peter C E.

Afiliação

Kwon H; School of Chemistry, University of Bristol, Bristol, United Kingdom. Electronic address: hanna.kwon@bristol.ac.uk.
Schrader TE; Forschungszentrum Jülich GmbH, Jülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ), Garching, Germany.
Ostermann A; Heinz Maier-Leibnitz Zentrum (MLZ), Technische Universität München, Garching, Germany.
Blakeley MP; Large Scale Structures Group, Institut Laue-Langevin, Grenoble, France.
Raven EL; School of Chemistry, University of Bristol, Bristol, United Kingdom.
Moody PCE; Department of Molecular and Cell Biology and Leicester Institute of Structural and Chemical Biology, University of Leicester, Leicester, United Kingdom.

Methods Enzymol ; 634: 379-389, 2020.

Article em En | MEDLINE | ID: mdl-32093841

ABSTRACT

ABSTRACT

By combining the normal practice for X-ray crystallography of collecting diffraction data at 100K with neutron crystallography the structures of cryo-trapped enzyme intermediates have been determined, revealing the positions of the previously hidden hydrogens that are essential to a better understanding of the involved mechanism.

Assuntos

Difração de Nêutrons; Nêutrons; Cristalografia; Cristalografia por Raios X; Heme; Peroxidases

Palavras-chave

Cryo-trapping; Enzyme intermediate; Heme peroxidase; Neutron diffraction; Protonation state

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Difração de Nêutrons / Nêutrons Idioma: En Revista: Methods Enzymol Ano de publicação: 2020 Tipo de documento: Article

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