In vitro effect of some nucleoside reverse transcriptase inhibitors against HSV-1 replication.
Eur Rev Med Pharmacol Sci
; 24(3): 1454-1459, 2020 02.
Article
em En
| MEDLINE
| ID: mdl-32096195
ABSTRACT
OBJECTIVE:
The current study was designed to investigate the effects of some nucleoside reverse transcriptase inhibitors (NRTIs) on HSV-1 infection. MATERIALS ANDMETHODS:
Initially, the SwissTargetPrediction server was used to predict the interactions between HSV-1 thymidine kinase and acyclovir, stavudine, zidovudine, didanosine, and entecavir. The effect of each component on Vero cell viability was assessed by the MTT assay. After treatment, the cell supernatants were collected, and HSV-1 replication was analyzed by quantitative real-time PCR.RESULTS:
The qPCR results revealed that viral titers were reduced 41, 40, 19, 44, and 31-fold in the presence of acyclovir, zidovudine, stavudine, didanosine, and entecavir, respectively.CONCLUSIONS:
Our findings indicate that NRTIs significantly reduce HSV-1 replication in cell culture.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antivirais
/
Replicação Viral
/
Herpesvirus Humano 1
/
Inibidores da Transcriptase Reversa
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Eur Rev Med Pharmacol Sci
Assunto da revista:
FARMACOLOGIA
/
TOXICOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Irã