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IP3 R2 null mice display a normal acquisition of somatic and neurological development milestones.
Guerra-Gomes, Sónia; Cunha-Garcia, Daniela; Marques Nascimento, Diana Sofia; Duarte-Silva, Sara; Loureiro-Campos, Eduardo; Morais Sardinha, Vanessa; Viana, João Filipe; Sousa, Nuno; Maciel, Patrícia; Pinto, Luísa; Oliveira, João Filipe.
Afiliação
  • Guerra-Gomes S; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.
  • Cunha-Garcia D; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
  • Marques Nascimento DS; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.
  • Duarte-Silva S; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
  • Loureiro-Campos E; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.
  • Morais Sardinha V; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
  • Viana JF; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.
  • Sousa N; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
  • Maciel P; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.
  • Pinto L; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
  • Oliveira JF; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.
Eur J Neurosci ; 54(5): 5673-5686, 2021 09.
Article em En | MEDLINE | ID: mdl-32166822
ABSTRACT
Astrocytes are key players in the regulation of brain development and function. They sense and respond to the surrounding activity by elevating their intracellular calcium (Ca2+ ) levels. These astrocytic Ca2+ elevations emerge from different sources and display complex spatio-temporal properties. Ca2+ elevations are spatially distributed in global (soma and main processes) and/or focal regions (microdomains). The inositol 1,4,5-trisphosphate receptor type 2 knockout (IP3 R2 KO) mouse model lacks global Ca2+ elevations in astrocytes, and it has been used by different laboratories. However, the constitutive deletion of IP3 R2 during development may trigger compensating phenotypes, which could bias the results of experiments using developing or adult mice. To address this issue, we performed a detailed neurodevelopmental evaluation of male and female IP3 R2 KO mice, during the first 21 days of life, as well as an evaluation of motor function, strength and neurological reflexes in adult mice. Our results show that male and female IP3 R2 KO mice display a normal acquisition of developmental milestones, as compared with wild-type (WT) mice. We also show that IP3 R2 KO mice display normal motor coordination, strength and neurological reflexes in adulthood. To exclude a potential compensatory overexpression of other IP3 Rs, we quantified the relative mRNA levels of all 3 subtypes, in brain tissue. We found that, along with the complete deletion of Itpr2, there is no compensatory expression of Itpr1 or Itrp3. Overall, our results show that the IP3 R2 KO mouse is a reliable model to study the functional impact of global IP3 R2-dependent astrocytic Ca2+ elevations.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Astrócitos / Sinalização do Cálcio Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Eur J Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Portugal

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Astrócitos / Sinalização do Cálcio Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Eur J Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Portugal