First steps towards combining faecal immunochemical testing with the gut microbiome in colorectal cancer screening.
United European Gastroenterol J
; 8(3): 293-302, 2020 04.
Article
em En
| MEDLINE
| ID: mdl-32213018
ABSTRACT
OBJECTIVES:
Many countries use faecal immunochemical testing (FIT) to screen for colorectal cancer. There is increasing evidence that faecal microbiota play a crucial role in colorectal cancer carcinogenesis. We assessed the possibility of measuring faecal microbial features in FIT as potential future biomarkers in colorectal cancer screening.METHODS:
Bacterial stability over time and the possibility of bacterial contamination were evaluated using quantitative polymerase chain reaction analysis. Positive FIT samples (n = 200) of an average-risk screening cohort were subsequently analysed for universal 16S, and bacteria. Escherichia coli (E. coli), Fusobacterium nucleatum (F. nucleatum), Bacteroidetes and Faecalibacterium prausnitzii (F. prausnitzii) by qPCR. The results were compared with colonoscopy findings.RESULTS:
Faecal microbiota in FIT were stably measured up to six days for E. coli (p = 0.53), F. nucleatum (p = 0.30), Bacteroidetes (p = 0.05) and F. prausnitzii (p = 0.62). Overall presence of bacterial contamination in FIT controls was low. Total bacterial load (i.e. 16S) was significantly higher in patients with colorectal cancer and high-grade dysplasia (p = 0.006). For the individual bacteria tested, no association was found with colonic lesions.CONCLUSIONS:
These results show that the faecal microbial content can be measured in FIT samples and remains stable for six days. Total bacterial load was higher in colorectal cancer and high-grade dysplasia. These results pave the way for further research to determine the potential role of microbiota assessment in FIT screening.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Colorretais
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Programas de Rastreamento
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Detecção Precoce de Câncer
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Fezes
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Microbioma Gastrointestinal
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
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Screening_studies
País/Região como assunto:
Europa
Idioma:
En
Revista:
United European Gastroenterol J
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Holanda