Translation of insulin granule proteins are regulated by PDI and PABP.
Biochem Biophys Res Commun
; 526(3): 618-625, 2020 06 04.
Article
em En
| MEDLINE
| ID: mdl-32248978
ABSTRACT
Glucose mediated insulin biosynthesis is tightly regulated and shared between insulin granule proteins such as its processing enzymes, prohormone convertases, PC1/3 and PC2. However, the molecular players involved in the co-ordinated translation remain elusive. The trans-acting factors like PABP (Poly A Binding Protein) and PDI (Protein Disulphide Isomerize) binds to a conserved sequence in the 5'UTR of insulin mRNA and regulates its translation. Here, we demonstrate that 5'UTR of PC1/3 and PC2 also associate with PDI and PABP. We show that a' and RRM 3-4 domains of PDI and PABP respectively, are necessary for RNA binding activity to the 5'UTRs of insulin and its processing enzymes.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
/
Isomerases de Dissulfetos de Proteínas
/
Proteínas de Ligação a Poli(A)
/
Pró-Proteína Convertase 1
/
Pró-Proteína Convertase 2
/
Insulina
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Índia