Intestinal microbiota regulates tryptophan metabolism following oral infection with Toxoplasma gondii.
Parasite Immunol
; 42(9): e12720, 2020 09.
Article
em En
| MEDLINE
| ID: mdl-32275066
ABSTRACT
INTRODUCTION:
The intestinal microbiota plays an important role in modulating host immune responses. Oral Toxoplasma gondii infection can promote intestinal inflammation in certain mice strains. The IDO-AhR axis may control tryptophan (Trp) metabolism constituting an important immune regulatory mechanism in inflammatory settings.AIMS:
In the present study, we investigated the role of the intestinal microbiota on Trp metabolism during oral infection with T gondii. METHODS ANDRESULTS:
Mice were treated with antibiotics for four weeks and then infected with T gondii by gavage. Histopathology and immune responses were evaluated 8 days after infection. We found that depletion of intestinal microbiota by antibiotics contributed to resistance against T gondii infection and led to reduced expression of AhR on dendritic and Treg cells. Mice depleted of Gram-negative bacteria presented higher levels of systemic Trp, downregulation of AhR expression and increased resistance to infection whereas depletion of Gram-positive bacteria did not affect susceptibility or expression of AhR on immune cells.CONCLUSION:
Our findings indicate that the intestinal microbiota can control Trp availability and provide a link between the AhR pathway and host-microbiota interaction in acute infection with T gondii.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Triptofano
/
Toxoplasmose
/
Microbioma Gastrointestinal
Limite:
Animals
Idioma:
En
Revista:
Parasite Immunol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Brasil