Evolutionary trade-offs of insecticide resistance - The fitness costs associated with target-site mutations in the nAChR of Drosophila melanogaster.
Mol Ecol
; 29(14): 2661-2675, 2020 07.
Article
em En
| MEDLINE
| ID: mdl-32510730
ABSTRACT
The evolution of resistance to drugs and pesticides poses a major threat to human health and food security. Neonicotinoids are highly effective insecticides used to control agricultural pests. They target the insect nicotinic acetylcholine receptor and mutations of the receptor that confer resistance have been slow to develop, with only one field-evolved mutation being reported to date. This is an arginine-to-threonine substitution at position 81 of the nAChR_ß1 subunit in neonicotinoid-resistant aphids. To validate the role of R81T in neonicotinoid resistance and to test whether it may confer any significant fitness costs to insects, CRISPR/Cas9 was used to introduce an analogous mutation in the genome of Drosophila melanogaster. Flies carrying R81T showed an increased tolerance (resistance) to neonicotinoid insecticides, accompanied by a significant reduction in fitness. In comparison, flies carrying a deletion of the whole nAChR_α6 subunit, the target site of spinosyns, showed an increased tolerance to this class of insecticides but presented almost no fitness deficits.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resistência a Inseticidas
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Receptores Nicotínicos
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Proteínas de Drosophila
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Drosophila melanogaster
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Aptidão Genética
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Neonicotinoides
Tipo de estudo:
Health_economic_evaluation
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Mol Ecol
Assunto da revista:
BIOLOGIA MOLECULAR
/
SAUDE AMBIENTAL
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Reino Unido