Your browser doesn't support javascript.
loading
Comparative transcriptomic analysis of resistant and susceptible tea cultivars in response to Empoasca onukii (Matsuda) damage.
Jin, Shan; Ren, Qianqian; Lian, Lingli; Cai, Xiaoming; Bian, Lei; Luo, Zongxiu; Li, Zhaoqun; Ye, Naixing; Wei, Rifeng; He, Weiyi; Liu, Wei; Chen, Zongmao.
Afiliação
  • Jin S; Key Laboratory of Tea Science of Fujian Province, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Ren Q; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China.
  • Lian L; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Cai X; Key Laboratory of Tea Science of Fujian Province, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Bian L; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Luo Z; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China.
  • Li Z; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China.
  • Ye N; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China.
  • Wei R; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China.
  • He W; Key Laboratory of Tea Science of Fujian Province, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Liu W; Key Laboratory of Tea Science of Fujian Province, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Chen Z; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Planta ; 252(1): 10, 2020 Jun 29.
Article em En | MEDLINE | ID: mdl-32601995
ABSTRACT
MAIN

CONCLUSION:

Transcriptomic studies in resistant and susceptible tea cultivars have been performed to reveal the different defense molecular mechanisms of tea after E. onukii feeding. The molecular mechanism by which tea plants respond to small green leafhopper Empoasca onukii (Matsuda) damage is unclear. Using the resistant tea plant cultivar Juyan (JY) and the susceptible tea plant cultivar Enbiao (EB) as materials, this study performed RNA-seq on tea leaf samples collected at three time points (6 h, 12 h, 24 h) during exposure of the plants to leafhopper to reveal the molecular mechanisms that are activated in susceptible and resistant tea plant cultivars in response to leafhopper damage. The numbers of DEGs in the susceptible tea cultivar during early (6 h) and late (24 h) stages of leafhopper induction were higher than those in the resistant cultivar at the same time points. The stress responses to leafhopper were most intense at 12 h in both tea cultivars. Pathway enrichment analysis showed that most up-regulated DEGs and their related metabolic pathways were similar in the two tea cultivars. However, during the early stage of leafhopper induction (6 h), jasmonic acid (JA)-related genes were significantly up-regulated in the resistant cultivar. The terpenoid biosynthetic pathway and the α-linolenic acid metabolic pathway were activated earlier in the resistant cultivar and remained activated until the late stage of leafhopper damage. Our results confirmed that after leafhopper damage, the resistant tea cultivar activated its defense responses earlier than the susceptible cultivar, and these defense responses were mainly related to terpenoid metabolism and JA biosynthetic pathway. The results provide important clues for further studies on resistance strategy of tea plants to pest.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Camellia sinensis / Resistência à Doença / Transcriptoma / Hemípteros Limite: Animals Idioma: En Revista: Planta Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Plantas / Camellia sinensis / Resistência à Doença / Transcriptoma / Hemípteros Limite: Animals Idioma: En Revista: Planta Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China