Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2.
Emerg Infect Dis
; 26(10): 2401-2405, 2020 Oct.
Article
em En
| MEDLINE
| ID: mdl-32610037
ABSTRACT
We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pneumonia Viral
/
RNA Viral
/
Análise de Sequência de RNA
/
Infecções por Coronavirus
/
Betacoronavirus
/
Sequenciamento Completo do Genoma
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Emerg Infect Dis
Assunto da revista:
DOENCAS TRANSMISSIVEIS
Ano de publicação:
2020
Tipo de documento:
Article