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Combining genome composition and differential gene expression analyses reveals that SmPGH1 contributes to bacterial wilt resistance in somatic hybrids.
Wang, Haibo; Cheng, Zhengnan; Wang, Bingsen; Dong, Jianke; Ye, Wenxuan; Yu, Yan; Liu, Ting; Cai, Xingkui; Song, Botao; Liu, Jun.
Afiliação
  • Wang H; Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
  • Cheng Z; Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, China.
  • Wang B; National Center for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, 430070, China.
  • Dong J; Huazhong Agricultural University, Wuhan, 43070, China.
  • Ye W; Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
  • Yu Y; Huazhong Agricultural University, Wuhan, 43070, China.
  • Liu T; Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, 430070, China.
  • Cai X; Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, China.
  • Song B; National Center for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, 430070, China.
  • Liu J; Huazhong Agricultural University, Wuhan, 43070, China.
Plant Cell Rep ; 39(9): 1235-1248, 2020 Sep.
Article em En | MEDLINE | ID: mdl-32666195
KEY MESSAGE: Clarification of the genome composition of the potato + eggplant somatic hybrids cooperated with transcriptome analysis efficiently identified the eggplant gene SmPGH1 that contributes to bacterial wilt resistance. The cultivated potato is susceptible and lacks resistance to bacterial wilt (BW), a soil-borne disease caused by Ralstonia solanacearum. It also has interspecies incompatibility within Solanaceae plants. Previously, we have successfully conducted the protoplast fusion of potato and eggplant and regenerated somatic hybrids that showing resistance to eggplant BW. For efficient use of these novel germplasm and improve BW resistance of cultivated potato, it is essential to dissect the genetic basis of the resistance to BW obtained from eggplant. The strategy of combining genome composition and transcriptome analysis was established to explore the gene that confers BW resistance to the hybrids. Genome composition of the 90 somatic hybrids was studied using genomic in situ hybridization coupled with 44 selected eggplant-specific SSRs (smSSRs). The analysis revealed a diverse set of genome combinations among the hybrids and showed a possibility of integration of alien genes along with the detection of 7 smSSRs linked to BW resistance (BW-linked SSRs) in the hybrids. Transcriptome comparison between the resistant and susceptible gene pools identified a BW resistance associated gene, smPGH1, which was significantly induced by R. solanacearum in the resistant pool. Remarkably, smPGH1 was co-localized with the BW-linked SSR emh01E15 on eggplant chromosome 9, which was further confirmed that smPGH1 was activated by R. solanacearum only in the resistant hybrids. Taken together, the identified gene smPGH1 and BW-linked SSRs have provided novel genetic resources that will aid in potato breeding for BW resistance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Solanum tuberosum / Genoma de Planta / Solanum melongena / Resistência à Doença Idioma: En Revista: Plant Cell Rep Assunto da revista: BOTANICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Solanum tuberosum / Genoma de Planta / Solanum melongena / Resistência à Doença Idioma: En Revista: Plant Cell Rep Assunto da revista: BOTANICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China