Quantitation of 2-hydroxyglutarate in human plasma via LC-MS/MS using a surrogate analyte approach.
Bioanalysis
; 12(16): 1149-1159, 2020 Aug.
Article
em En
| MEDLINE
| ID: mdl-32757862
ABSTRACT
Aim:
2-Hydroxyglutarate (2-HG) is a target engagement biomarker in patients after treatment with inhibitors of mutated isocitrate dehydrogenase (mIDH). Accurate measurement of 2-HG is critical for monitoring the inhibition effectiveness of the inhibitors. Materials &methods:
Human plasma samples were spiked with stable isotope labelled internal standard, processed by protein precipitation, and analyzed using LC-MS/MS. This method was validated following regulatory guidance and has been successfully applied in a clinical study for mIDH inhibition.Results:
An LC-MS/MS method with a surrogate analyte approach was developed and validated to measure 2-HG in human plasma with acceptable intra- and inter-assay accuracy and precision.Conclusion:
A sensitive and robust LC-MS/MS method was developed and validated for measuring 2-HG in human plasma.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida
/
Espectrometria de Massas em Tandem
/
Glutaratos
Tipo de estudo:
Guideline
Limite:
Humans
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Estados Unidos