GTP gamma S activation of proto-oncogene expression in transiently permeabilised Swiss 3T3 fibroblasts.
FEBS Lett
; 227(2): 203-8, 1988 Jan 25.
Article
em En
| MEDLINE
| ID: mdl-3276558
ABSTRACT
A technique of transient permeabilisation has been used to show that the introduction of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), a non-hydrolysable analogue of GTP, into intact Swiss 3T3 fibroblasts stimulates phosphoinositide hydrolysis, cyclic AMP accumulation and the activation of c-fos and c-myc proto-oncogenes. Of a number of nucleotide triphosphates introduced into the cells, only GTP and its non-hydrolysable analogues activated inositol phosphate release, suggesting that this response is mediated by guanine nucleotide regulatory (G) protein(s). The data demonstrate that transient permeabilisation provides a method of examining the involvement of G-proteins in nuclear activation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tionucleotídeos
/
Transcrição Gênica
/
Proto-Oncogenes
/
Guanosina Trifosfato
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
Reino Unido