Internal morphological changes during metamorphosis in the sheep nasal bot fly, Oestrus ovis.
Med Vet Entomol
; 34(4): 476-487, 2020 12.
Article
em En
| MEDLINE
| ID: mdl-32767606
ABSTRACT
During the larval stage, oestrid flies (Diptera Oestridae) are obligate parasites, whereas during the adult stage they are free-living and do not feed. Like other cyclorrhaphous flies, oestrids undergo metamorphosis inside an opaque puparium, formed by the contracted and hardened cuticle of the third-instar larva. The present study documents the internal morphological changes taking place during metamorphosis of the sheep nasal bot fly, Oestrus ovis L., using non-invasive, micro-CT-based virtual histology and provides quantitative data of volumetric changes in specific organs. Virtual histological sections allowed visualisation of the progression and completion of the apolyses, which delimit the different intra-puparial stages, and the connection to the tracheal system of a large gas bubble, which plays an essential role during early metamorphosis. Overall, our results show that the sequence of morphological and volumetric changes in tissues and organs is similar to those found in other cyclorrhaphous flies, but they also reveal developmental differences that result in an adult vestigial digestive tract. Future studies could develop non-invasive, reliable methods for aging the intra-puparial forms of different oestrid species of veterinary importance, based on both qualitative and quantitative markers, thus improving our knowledge of their development and the efficiency of control strategies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dípteros
/
Miíase
Tipo de estudo:
Qualitative_research
Limite:
Animals
Idioma:
En
Revista:
Med Vet Entomol
Assunto da revista:
BIOLOGIA
/
MEDICINA VETERINARIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Espanha