Characterization of anti-BCG benz[α]anthraquinones and new siderophores from a Xinjiang desert-isolated rare actinomycete Nocardia sp. XJ31.
Appl Microbiol Biotechnol
; 104(19): 8267-8278, 2020 Oct.
Article
em En
| MEDLINE
| ID: mdl-32830291
ABSTRACT
The current global demand for novel anti-TB drugs has drawn urgent attention on the discovery of natural product compounds with anti-TB activity. Lots of efforts have emphasized on environmental samples from unexplored or underexplored natural habits and identified numerous rare actinomycete taxa producing structurally diverse bioactive natural products. Herein, we report a survey of the rare actinobacteria diversity in Xinjiang region together with the discovery of anti-TB active natural products from these strains. We have collected 17 soil samples at different sites with different environmental conditions, from which 39 rare actinobacteria were identified by using a selective isolation strategy with 5 media variations. Among those isolated strains, XJ31 was identified as a new Nocardia sp. based on 16S rRNA gene analysis. Through one strain-many compounds (OSMAC) strategy combined with anti-Bacillus Calmette-Guérin bioassay-guided isolation, two groups of compounds were identified. They were twelve siderophores (nocardimicins, 1-12) and two anthraquinones (brasiliquinones, 13 and 14) and ten of them were identified as new compounds. The structures of the purified compounds were elucidated using HR-ESI-MS, 1D NMR, and 2D NMR techniques. The anti-TB bioassays revealed that the two benz[α]anthraquinones have potent activity against BCG (MICs = 25 µM), which can be used as a promising start point for further anti-TB drug development. KEY POINTS ⢠Ten new natural products were identified from Nocardia sp. XJ31. ⢠Brasiliquinones 13 and 14 showed moderate anti-BCG activity.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Actinobacteria
/
Nocardia
Tipo de estudo:
Qualitative_research
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China