Kinetic profiling of metabolic specialists demonstrates stability and consistency of in vivo enzyme turnover numbers.

Heckmann, David; Campeau, Anaamika; Lloyd, Colton J; Phaneuf, Patrick V; Hefner, Ying; Carrillo-Terrazas, Marvic; Feist, Adam M; Gonzalez, David J; Palsson, Bernhard O

Heckmann, David; Campeau, Anaamika; Lloyd, Colton J; Phaneuf, Patrick V; Hefner, Ying; Carrillo-Terrazas, Marvic; Feist, Adam M; Gonzalez, David J; Palsson, Bernhard O.

Afiliação

Heckmann D; Department of Bioengineering, University of California San Diego, La Jolla, CA 92093; david.heckmann@hhu.de palsson@ucsd.edu.
Campeau A; Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093.
Lloyd CJ; Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.
Phaneuf PV; Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.
Hefner Y; Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.
Carrillo-Terrazas M; Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093.
Feist AM; Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.
Gonzalez DJ; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Lyngby, Denmark.
Palsson BO; Department of Pharmacology, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093.

Proc Natl Acad Sci U S A ; 117(37): 23182-23190, 2020 09 15.

Article em En | MEDLINE | ID: mdl-32873645

Assuntos

Escherichia coli/metabolismo; Escherichia coli/genética; Proteínas de Escherichia coli/genética; Proteínas de Escherichia coli/metabolismo; Técnicas de Inativação de Genes/métodos; Genoma/genética; Cinética; Aprendizado de Máquina; Modelos Biológicos; Proteômica/métodos

Palavras-chave

gene knockout; in vivo; kcat; proteomics; turnover number

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Escherichia coli Tipo de estudo: Prognostic_studies Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2020 Tipo de documento: Article

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