Ribosomal Elongation of Aminobenzoic Acid Derivatives.
J Am Chem Soc
; 142(39): 16518-16522, 2020 09 30.
Article
em En
| MEDLINE
| ID: mdl-32946698
ABSTRACT
2-Aminobenzoic acid and heterocyclic amino acids are aromatic homologues of ß-amino acids, but their chemical properties are quite distinct. Because of the poor nucleophilicity of the amino group conjugated with the aromatic ring, no literature reports of their successful ribosomal elongation in nascent peptide chains have appeared to date. Here we report for the first time their incorporation in nascent peptide chains by means of a reconstituted translation system in which a designer tRNAPro1E2 capable of recruiting the elongation factors EF-Tu and EF-P was charged with their derivatives and the corresponding peptides were successfully expressed. We also demonstrate the expression of macrocyclic peptides containing exotic amino acids, including aminobenzoic acid derivatives.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Ribossomos
/
Aminobenzoatos
Idioma:
En
Revista:
J Am Chem Soc
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Japão