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Standardization of type 1 and type 2 diabetic nephropathy models in rats: Assessment and characterization of metabolic features and renal injury.
Kaikini, Aakruti A; Dhodi, Divya; Muke, Suraj; Peshattiwar, Vaibhavi; Bagle, Sneha; Korde, Aruna; Sarnaik, Jayula; Kadwad, Vijay; Sachdev, Satbir; Sathaye, Sadhana.
Afiliação
  • Kaikini AA; Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, Maharashtra, India.
  • Dhodi D; Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, Maharashtra, India.
  • Muke S; Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, Maharashtra, India.
  • Peshattiwar V; Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, Maharashtra, India.
  • Bagle S; Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, Maharashtra, India.
  • Korde A; Radiopharmaceuticals Programme, Board of Radiation & Isotope Technology (BRIT), Navi Mumbai, Maharashtra, India.
  • Sarnaik J; Radiopharmaceuticals Programme, Board of Radiation & Isotope Technology (BRIT), Navi Mumbai, Maharashtra, India.
  • Kadwad V; Radiopharmaceuticals Programme, Board of Radiation & Isotope Technology (BRIT), Navi Mumbai, Maharashtra, India.
  • Sachdev S; Radiopharmaceuticals Programme, Board of Radiation & Isotope Technology (BRIT), Navi Mumbai, Maharashtra, India.
  • Sathaye S; Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, Maharashtra, India.
J Pharm Bioallied Sci ; 12(3): 295-307, 2020.
Article em En | MEDLINE | ID: mdl-33100790
ABSTRACT

BACKGROUND:

Diabetes mellitus and its complications, such as nephropathy, represent a global burden. Recent research focuses on developing drugs that specifically target the pathogenesis of diabetic nephropathy rather than merely treating hyperglycemia. Rodent models of animal disease are integral in drug discovery and represent an obligatory regulatory requirement.

AIM:

The aim of this study was to develop and standardize rat models of type 1 and type 2 diabetic nephropathy, resembling characteristics of human clinical condition. MATERIALS AND

METHODS:

Rats were administered streptozotocin (STZ) 50 mg/kg intraperitoneally (i.p.), and STZ 50 mg/kg i.p. + nicotinamide (NA) 110 mg/kg i.p., for induction of type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM), respectively. Metabolic parameters (body weight, feed and water intake, blood glucose, serum insulin, oral glucose tolerance test, intraperitoneal insulin tolerance test, and indices of insulin sensitivity) were evaluated to characterize the symptoms of T1DM and T2DM. Renal damage was confirmed by the estimation of renal function biomarkers, kidney antioxidant status, kidney hypertrophy index, and histopathology.

RESULTS:

STZ and STZ + NA administration increased blood glucose levels significantly. Metabolic parameters indicated that administration of STZ resulted in clinical features of human T1DM, whereas STZ + NA rats resembled human T2DM. STZ- and STZ + NA-treated rats developed diabetic nephropathy in 4 weeks, indicated by altered levels of renal function markers, increased kidney hypertrophy index, increased renal oxidative stress, and altered tissue architecture. The study proposes reproducible and cost-effective rat models for both T1DM- and T2DM-induced diabetic nephropathy characterized by stable metabolic features and typical renal lesions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: J Pharm Bioallied Sci Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Índia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: J Pharm Bioallied Sci Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Índia