Expression and purification of active human kinases using Pichia pastoris as a general-purpose host.

Abdel Aziz, May H; Fan, Yao; Liu, Lijun; Moasser, Mark M; Fu, Haian; Jura, Natalia; Arkin, Michelle R

Abdel Aziz, May H; Fan, Yao; Liu, Lijun; Moasser, Mark M; Fu, Haian; Jura, Natalia; Arkin, Michelle R.

Afiliação

Abdel Aziz MH; Small Molecule Discovery Center, Department of Pharmaceutical Chemistry and Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, CA, USA. Electronic address: mabdelaziz@uttyler.edu.
Fan Y; Small Molecule Discovery Center, Department of Pharmaceutical Chemistry and Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
Liu L; Cardiovascular Research Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA, USA.
Moasser MM; Department of Medicine and Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
Fu H; Department of Pharmacology and Chemical Biology and Emory Chemical Biology Discovery Center, Emory University School of Medicine and Winship Cancer Institute, Atlanta, GA, USA.
Jura N; Cardiovascular Research Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA, USA.
Arkin MR; Small Molecule Discovery Center, Department of Pharmaceutical Chemistry and Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, CA, USA. Electronic address: michelle.arkin@ucsf.edu.

Protein Expr Purif ; 179: 105780, 2021 03.

Article em En | MEDLINE | ID: mdl-33115654

Assuntos

Proteínas Quinases; Proteínas Recombinantes de Fusão; Saccharomycetales; Animais; Cromatografia de Afinidade; Humanos; Domínios Proteicos/genética; Proteínas Quinases/genética; Proteínas Quinases/isolamento & purificação; Proteínas Quinases/metabolismo; Proteínas Recombinantes de Fusão/genética; Proteínas Recombinantes de Fusão/isolamento & purificação; Proteínas Recombinantes de Fusão/metabolismo; Saccharomycetales/genética; Saccharomycetales/metabolismo; Células Sf9; Solubilidade

Palavras-chave

Activity; Expression; Kinase; P. pastoris; Yeast

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Quinases / Proteínas Recombinantes de Fusão / Saccharomycetales Limite: Animals / Humans Idioma: En Revista: Protein Expr Purif Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2021 Tipo de documento: Article

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