Studies on the sequence and structure of the Escherichia coli K-12 nupG gene, encoding a nucleoside-transport system.
Eur J Biochem
; 168(2): 385-91, 1987 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-3311747
ABSTRACT
The nupG gene, encoding one of the two active nucleoside-transport systems in Escherichia coli K-12, has been cloned on the multicopy plasmid pBR322 and derivatives thereof. The recombinant plasmids complemented a chromosomal nupG mutation. A genetic map was determined by digestion with restriction endonucleases and the nucleotide sequence of a 3-kb stretch of DNA has been determined on fragments cloned into M13 phages. An open reading frame of 1254 bp, encoding a protein with a calculated molecular mass of 45.333 kDa, was deduced to be the coding region of nupG. Minicell-forming strains carrying plasmids containing this gene were shown to produce a hydrophobic, membrane-bound polypeptide with an apparent molecular mass of approximately 43 kDa.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Escherichia coli
/
Genes
/
Nucleosídeos
Idioma:
En
Revista:
Eur J Biochem
Ano de publicação:
1987
Tipo de documento:
Article
País de afiliação:
Dinamarca