Ubiquitinome Profiling Reveals the Landscape of Ubiquitination Regulation in Rice Young Panicles.
Genomics Proteomics Bioinformatics
; 18(3): 305-320, 2020 06.
Article
em En
| MEDLINE
| ID: mdl-33147495
ABSTRACT
Ubiquitination, an essential post-transcriptional modification (PTM), plays a vital role in nearly every biological process, including development and growth. Despite its functions in plant reproductive development, its targets in rice panicles remain unclear. In this study, we used proteome-wide profiling of lysine ubiquitination in rice (O. sativa ssp. indica) young panicles. We created the largest ubiquitinome dataset in rice to date, identifying 1638 lysine ubiquitination sites on 916 unique proteins. We detected three conserved ubiquitination motifs, noting that acidic glutamic acid (E) and aspartic acid (D) were most frequently present around ubiquitinated lysine. Enrichment analysis of Gene Ontology (GO) annotations and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of these ubiquitinated proteins revealed that ubiquitination plays an important role in fundamental cellular processes in rice young panicles. Interestingly, enrichment analysis of protein domains indicated that ubiquitination was enriched on a variety of receptor-like kinases and cytoplasmic tyrosine and serine-threonine kinases. Furthermore, we analyzed the crosstalk between ubiquitination, acetylation, and succinylation, and constructed a potential protein interaction network within our rice ubiquitinome. Moreover, we identified ubiquitinated proteins related to pollen and grain development, indicating that ubiquitination may play a critical role in the physiological functions in young panicles. Taken together, we reported the most comprehensive lysine ubiquitinome in rice so far, and used it to reveal the functional role of lysine ubiquitination in rice young panicles.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
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Oryza
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Processamento de Proteína Pós-Traducional
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Proteoma
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Ubiquitina
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Ubiquitinação
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Lisina
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Genomics Proteomics Bioinformatics
Assunto da revista:
BIOQUIMICA
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GENETICA
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INFORMATICA MEDICA
Ano de publicação:
2020
Tipo de documento:
Article