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Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2.
Custódio, Tânia F; Das, Hrishikesh; Sheward, Daniel J; Hanke, Leo; Pazicky, Samuel; Pieprzyk, Joanna; Sorgenfrei, Michèle; Schroer, Martin A; Gruzinov, Andrey Yu; Jeffries, Cy M; Graewert, Melissa A; Svergun, Dmitri I; Dobrev, Nikolay; Remans, Kim; Seeger, Markus A; McInerney, Gerald M; Murrell, Ben; Hällberg, B Martin; Löw, Christian.
Afiliação
  • Custódio TF; Centre for Structural Systems Biology (CSSB), DESY and European Molecular Biology Laboratory Hamburg, Notkestrasse 85, D-22607, Hamburg, Germany.
  • Das H; Centre for Structural Systems Biology (CSSB) and Karolinska Institutet VR-RÅC, Notkestrasse 85, D-22607, Hamburg, Germany.
  • Sheward DJ; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, 17177, Sweden.
  • Hanke L; Division of Virology, Institute of Infectious Diseases and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa.
  • Pazicky S; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, 17177, Sweden.
  • Pieprzyk J; Centre for Structural Systems Biology (CSSB), DESY and European Molecular Biology Laboratory Hamburg, Notkestrasse 85, D-22607, Hamburg, Germany.
  • Sorgenfrei M; Centre for Structural Systems Biology (CSSB), DESY and European Molecular Biology Laboratory Hamburg, Notkestrasse 85, D-22607, Hamburg, Germany.
  • Schroer MA; Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
  • Gruzinov AY; European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen Synchrotron (DESY), Notkestrasse 85, D-22607, Hamburg, Germany.
  • Jeffries CM; European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen Synchrotron (DESY), Notkestrasse 85, D-22607, Hamburg, Germany.
  • Graewert MA; European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen Synchrotron (DESY), Notkestrasse 85, D-22607, Hamburg, Germany.
  • Svergun DI; European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen Synchrotron (DESY), Notkestrasse 85, D-22607, Hamburg, Germany.
  • Dobrev N; European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen Synchrotron (DESY), Notkestrasse 85, D-22607, Hamburg, Germany.
  • Remans K; European Molecular Biology Laboratory (EMBL) Heidelberg, Protein Expression and Purification Core Facility, 69117, Heidelberg, Germany.
  • Seeger MA; European Molecular Biology Laboratory (EMBL) Heidelberg, Protein Expression and Purification Core Facility, 69117, Heidelberg, Germany.
  • McInerney GM; Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
  • Murrell B; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, 17177, Sweden.
  • Hällberg BM; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, 17177, Sweden. benjamin.murrell@ki.se.
  • Löw C; Centre for Structural Systems Biology (CSSB) and Karolinska Institutet VR-RÅC, Notkestrasse 85, D-22607, Hamburg, Germany. martin.hallberg@ki.se.
Nat Commun ; 11(1): 5588, 2020 11 04.
Article em En | MEDLINE | ID: mdl-33149112
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia Viral / Infecções por Coronavirus / Peptidil Dipeptidase A / Pandemias / Anticorpos de Domínio Único / Glicoproteína da Espícula de Coronavírus / Betacoronavirus Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Alemanha
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia Viral / Infecções por Coronavirus / Peptidil Dipeptidase A / Pandemias / Anticorpos de Domínio Único / Glicoproteína da Espícula de Coronavírus / Betacoronavirus Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Alemanha