Analysis of canine myeloid-derived suppressor cells (MDSCs) utilizing fluorescence-activated cell sorting, RNA protection mediums to yield quality RNA for single-cell RNA sequencing.

Jackson, K; Milner, R J; Doty, A; Hutchison, S; Cortes-Hinojosa, G; Riva, A; Sahay, B; Lejeune, A; Bechtel, S

Jackson, K; Milner, R J; Doty, A; Hutchison, S; Cortes-Hinojosa, G; Riva, A; Sahay, B; Lejeune, A; Bechtel, S.

Afiliação

Jackson K; Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, United States.
Milner RJ; Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, United States. Electronic address: milnerr@ufl.edu.
Doty A; Flow Cytometry and Imaging Core Lab, Interdisciplinary Center for Biotechnology Research, University of Florida, United States.
Hutchison S; Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, United States.
Cortes-Hinojosa G; Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, United States.
Riva A; Bioinformatics Core, Interdisciplinary Center for Biotechnology Research, University of Florida, United States.
Sahay B; Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, United States.
Lejeune A; Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, United States.
Bechtel S; Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, United States.

Vet Immunol Immunopathol ; 231: 110144, 2021 Jan.

Article em En | MEDLINE | ID: mdl-33278779

Assuntos

Doenças do Cão/genética; Citometria de Fluxo/veterinária; Melanoma/veterinária; Neoplasias Bucais/veterinária; Células Supressoras Mieloides/metabolismo; Animais; Antígeno CD11b; Sobrevivência Celular; Cães; Citometria de Fluxo/métodos; Regulação Neoplásica da Expressão Gênica; Melanoma/genética; Neoplasias Bucais/genética; Preservação Biológica; RNA Neoplásico/isolamento & purificação; RNA Neoplásico/metabolismo; RNA-Seq/veterinária; Ribonucleases/metabolismo; Análise de Célula Única/veterinária

Palavras-chave

Canine; Flow cytometry; MDSC; Next generation sequencing; RNA isolation; Single-cell RNA sequencing

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Bucais / Doenças do Cão / Citometria de Fluxo / Células Supressoras Mieloides / Melanoma Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Revista: Vet Immunol Immunopathol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos

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