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Charting Extracellular Transcriptomes in The Human Biofluid RNA Atlas.
Hulstaert, Eva; Morlion, Annelien; Avila Cobos, Francisco; Verniers, Kimberly; Nuytens, Justine; Vanden Eynde, Eveline; Yigit, Nurten; Anckaert, Jasper; Geerts, Anja; Hindryckx, Pieter; Jacques, Peggy; Brusselle, Guy; Bracke, Ken R; Maes, Tania; Malfait, Thomas; Derveaux, Thierry; Ninclaus, Virginie; Van Cauwenbergh, Caroline; Roelens, Kristien; Roets, Ellen; Hemelsoet, Dimitri; Tilleman, Kelly; Brochez, Lieve; Kuersten, Scott; Simon, Lukas M; Karg, Sebastian; Kautzky-Willers, Alexandra; Leutner, Michael; Nöhammer, Christa; Slaby, Ondrej; Prins, Roméo Willinge; Koster, Jan; Lefever, Steve; Schroth, Gary P; Vandesompele, Jo; Mestdagh, Pieter.
Afiliação
  • Hulstaert E; Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; Department of Dermatology, Ghent University Hospital, C. Heymansla
  • Morlion A; Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Avila Cobos F; Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Verniers K; Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Nuytens J; Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Vanden Eynde E; Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Yigit N; Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Anckaert J; Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Geerts A; Department of Gastroenterology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Hindryckx P; Department of Gastroenterology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Jacques P; Department of Rheumatology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium; VIB Inflammation Research Center, Ghent University, Technologiepark 71, 9052 Ghent, Belgium.
  • Brusselle G; Department of Respiratory Medicine, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Bracke KR; Department of Respiratory Medicine, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Maes T; Department of Respiratory Medicine, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Malfait T; Department of Respiratory Medicine, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Derveaux T; Department of Ophthalmology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Ninclaus V; Department of Ophthalmology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Van Cauwenbergh C; Department of Ophthalmology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Roelens K; Department of Obstetrics, Women's Clinic, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Roets E; Department of Obstetrics, Women's Clinic, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Hemelsoet D; Department of Neurology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Tilleman K; Department of Reproductive Medicine, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Brochez L; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; Department of Dermatology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Kuersten S; Illumina, San Diego, CA, USA.
  • Simon LM; University of Texas Health Science Center, School of Biomedical Informatics, Center for Precision Health, Houston, TX, USA.
  • Karg S; Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Computational Biology, Neuherberg, Germany.
  • Kautzky-Willers A; Department of Internal Medicine III, Clinical Division of Endocrinology and Metabolism, Unit of Gender Medicine, Medical University of Vienna, Vienna, Austria.
  • Leutner M; Department of Internal Medicine III, Clinical Division of Endocrinology and Metabolism, Unit of Gender Medicine, Medical University of Vienna, Vienna, Austria.
  • Nöhammer C; Austrian Institute of Technology, Center for Health and Bioresources, Molecular Diagnostics, Vienna, Austria.
  • Slaby O; Masaryk Memorial Cancer Institute, Department of Comprehensive Cancer Care, Brno, Czech Republic; Department of Pathology, University Hospital Brno, Brno, Czech Republic; Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
  • Prins RW; Department of Oncogenomics, Amsterdam University Medical Centers (AUMC), University of Amsterdam, Amsterdam, the Netherlands.
  • Koster J; Department of Oncogenomics, Amsterdam University Medical Centers (AUMC), University of Amsterdam, Amsterdam, the Netherlands.
  • Lefever S; Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Schroth GP; Illumina, San Diego, CA, USA.
  • Vandesompele J; Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium.
  • Mestdagh P; Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium. Electronic address: pieter.mestdagh@ugent.be.
Cell Rep ; 33(13): 108552, 2020 12 29.
Article em En | MEDLINE | ID: mdl-33378673
ABSTRACT
Extracellular RNAs present in biofluids have emerged as potential biomarkers for disease. Where most studies focus on blood-derived fluids, other biofluids may be more informative. We present an atlas of messenger, circular, and small RNA transcriptomes of a comprehensive collection of 20 human biofluids. By means of synthetic spike-in controls, we compare RNA content across biofluids, revealing a 10,000-fold difference in concentration. The circular RNA fraction is increased in most biofluids compared to tissues. Each biofluid transcriptome is enriched for RNA molecules derived from specific tissues and cell types. Our atlas enables an informed selection of the most relevant biofluid to monitor particular diseases. To verify the biomarker potential in these biofluids, four validation cohorts representing a broad spectrum of diseases were profiled, revealing numerous differential RNAs between case and control subjects. Spike-normalized data are publicly available in the R2 web portal for further exploration.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Líquidos Corporais / RNA / Biomarcadores / Transcriptoma Tipo de estudo: Etiology_studies / Incidence_studies / Observational_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Cell Rep Ano de publicação: 2020 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Líquidos Corporais / RNA / Biomarcadores / Transcriptoma Tipo de estudo: Etiology_studies / Incidence_studies / Observational_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Cell Rep Ano de publicação: 2020 Tipo de documento: Article