Bovine NK-lysin-derived peptides have bactericidal effects against Mycobacterium avium subspecies paratuberculosis.
Vet Res
; 52(1): 11, 2021 Jan 21.
Article
em En
| MEDLINE
| ID: mdl-33478585
ABSTRACT
Infection with Mycobacterium avium subspecies paratuberculosis (MAP) is complex, but little is known about the role that natural killer (NK) cells play. In the present study, four bovine NK-lysin peptides were synthesized to evaluate their bactericidal activity against MAP. The results demonstrated that bNK-lysin peptides were directly bactericidal against MAP, with bNK1 and bNK2A being more potent than bNK2B and bNK2C. Mechanistically, transmission electron microscopy revealed that the incubation of MAP with bNK2A resulted in extensive damage to cell membranes and cytosolic content leakage. Furthermore, the addition of bNK2A linked with a cell-penetrating peptide resulted in increased MAP killing in a macrophage model.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteolipídeos
/
Mycobacterium avium subsp. paratuberculosis
/
Antibacterianos
Limite:
Animals
Idioma:
En
Revista:
Vet Res
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos