Distinct roles of LRP5 and LRP6 in Wnt signaling regulation in the retina.
Biochem Biophys Res Commun
; 545: 8-13, 2021 03 19.
Article
em En
| MEDLINE
| ID: mdl-33545636
ABSTRACT
Dysregulation of Wnt signaling is implicated in multiple ocular disorders. The roles of Wnt co-receptors LRP5 and LRP6 in Wnt signaling regulation remain elusive, as most retinal cells express both of the co-receptors. To address this question, LRP5 and LRP6 were individually knocked-out in a human retinal pigment epithelium cell line using the CRISPR-Cas9 technology. Wnt signaling activity induced by various Wnt ligands was measured using wild-type and the KO cell lines. The results identified three groups of Wnt ligands based on their co-receptor specificity 1) activation of Wnt signaling only through LRP6, 2) through both LRP5 and LRP6 and 3) predominantly through LRP5. These results indicate that LRP5 and LRP6 have differential roles in Wnt signaling regulation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Epitélio Pigmentado da Retina
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Proteína-5 Relacionada a Receptor de Lipoproteína de Baixa Densidade
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Proteína-6 Relacionada a Receptor de Lipoproteína de Baixa Densidade
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Via de Sinalização Wnt
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos