Anti-polyphenol oxidase properties of total flavonoids from young loquat fruits: inhibitory activity and mechanism.
Bioengineered
; 12(1): 640-647, 2021 12.
Article
em En
| MEDLINE
| ID: mdl-33587004
ABSTRACT
This study investigated anti-polyphenol oxidase activity and mechanism of purified total flavonoids (PTF) from young loquat fruits. PTF remarkably inhibited the activity of polyphenol oxidase (PPO) with an IC50 value of 21.03 ± 2.37 µg/mL. Based on enzyme kinetics, PTF was found to be a potent, mixed-type, and reversible inhibitor of PPO. The fluorescence intensity of PPO was quenched by PTF through forming a PTF-PPO complex in a static procedure. Therefore, this study authenticated PTF as an efficient PPO inhibitor, which would contribute to their utilization in food industry.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Flavonoides
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Catecol Oxidase
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Eriobotrya
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Inibidores Enzimáticos
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Frutas
Idioma:
En
Revista:
Bioengineered
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China