The effect of Q-switched 1064-nm Nd: YAG laser on skin barrier and collagen synthesis via miR-663a to regulate TGFß1/smad3/p38MAPK pathway.
Photodermatol Photoimmunol Photomed
; 37(5): 412-421, 2021 Sep.
Article
em En
| MEDLINE
| ID: mdl-33621359
ABSTRACT
BACKGROUND:
Our previous research found that Q-switched 1064-nm Nd YAG laser (1064-QSNYL) induces skin collagen synthesis by activating TGFß1/Smad3/p38MAPKs pathway. Moreover, a lot of studies shown that MicroRNAs (miRNAs) contribute to regulate collagen synthesis and skin barrier. Therefore, we intend to explore the mechanism of 1064-QSNYL on collagen synthesis and skin barrier through miRNAs.METHODS:
We predicted the upstream miRNAs of TGFß1 by bioinformatics databases, and verified them through dual-luciferase reporter genes and Western blotting. The expression of collagen, skin barrier-related protein K10 and filaggrin, TIMP-1, and MMP-2 were detected by RT-qPCR and Western blotting, respectively. Moreover, we detected moisture content, elasticity value, TEWL value, SOD vitality, and hydroxyproline content to evaluate skin barrier of mice. H&E staining to observe the change of dermis thickness and inflammation and infiltration of mice skin.RESULTS:
The results shown that TGFß1 was target gene of miR-663a. Moreover, we found that 1064-QSNYL activated TGFß1/smad3/p38MAPK pathway by down-regulating the expression of miR-663a in HaCaT, HDF cells, and mice, thereby promoting expression of Collagen I, Collagen IV, TIMP-1, K10, and filaggrin and inhibiting MMP-2. Furthermore, 1064-QSNYL contributed to moisture content, elasticity, SOD vitality, and hydroxyproline content via miR-663a to activate TGFß1/smad3/p38MAPK pathway.CONCLUSIONS:
In summary, this study found for the first time that 1064-QSNYL contributed to collagen synthesis and skin repair via miR-663a to regulate TGFß1/smad3/p38MAPK pathway, thereby achieving skin rejuvenation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
/
Lasers de Estado Sólido
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Photodermatol Photoimmunol Photomed
Assunto da revista:
ALERGIA E IMUNOLOGIA
/
DERMATOLOGIA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China