Your browser doesn't support javascript.
loading
Comparative Cell Surface Proteomic Analysis of the Primary Human T Cell and Monocyte Responses to Type I Interferon.
Soday, Lior; Potts, Martin; Hunter, Leah M; Ravenhill, Benjamin J; Houghton, Jack W; Williamson, James C; Antrobus, Robin; Wills, Mark R; Matheson, Nicholas J; Weekes, Michael P.
Afiliação
  • Soday L; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
  • Potts M; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
  • Hunter LM; Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom.
  • Ravenhill BJ; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
  • Houghton JW; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
  • Williamson JC; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
  • Antrobus R; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
  • Wills MR; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom.
  • Matheson NJ; Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom.
  • Weekes MP; Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), University of Cambridge, Cambridge, United Kingdom.
Front Immunol ; 12: 600056, 2021.
Article em En | MEDLINE | ID: mdl-33628210
The cellular response to interferon (IFN) is essential for antiviral immunity, IFN-based therapy and IFN-related disease. The plasma membrane (PM) provides a critical interface between the cell and its environment, and is the initial portal of entry for viruses. Nonetheless, the effect of IFN on PM proteins is surprisingly poorly understood, and has not been systematically investigated in primary immune cells. Here, we use multiplexed proteomics to quantify IFNα2a-stimulated PM protein changes in primary human CD14+ monocytes and CD4+ T cells from five donors, quantifying 606 and 482 PM proteins respectively. Comparison of cell surface proteomes revealed a remarkable invariance between donors in the overall composition of the cell surface from each cell type, but a marked donor-to-donor variability in the effects of IFNα2a. Furthermore, whereas only 2.7% of quantified proteins were consistently upregulated by IFNα2a at the surface of CD4+ T cells, 6.8% of proteins were consistently upregulated in primary monocytes, suggesting that the magnitude of the IFNα2a response varies according to cell type. Among these differentially regulated proteins, we found the viral target Endothelin-converting enzyme 1 (ECE1) to be an IFNα2a-stimulated protein exclusively upregulated at the surface of CD4+ T cells. We therefore provide a comprehensive map of the cell surface of IFNα2a-stimulated primary human immune cells, including previously uncharacterized interferon stimulated genes (ISGs) and candidate antiviral factors.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Monócitos / Linfócitos T CD4-Positivos / Interferon-alfa / Enzimas Conversoras de Endotelina Limite: Humans Idioma: En Revista: Front Immunol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Monócitos / Linfócitos T CD4-Positivos / Interferon-alfa / Enzimas Conversoras de Endotelina Limite: Humans Idioma: En Revista: Front Immunol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Reino Unido