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Rapid subcellular calcium responses and dynamics by calcium sensor G-CatchER.
Reddish, Florence N; Miller, Cassandra L; Deng, Xiaonan; Dong, Bin; Patel, Atit A; Ghane, Mohammad A; Mosca, Barbara; McBean, Cheyenne; Wu, Shengnan; Solntsev, Kyril M; Zhuo, You; Gadda, Giovanni; Fang, Ning; Cox, Daniel N; Mabb, Angela M; Treves, Susan; Zorzato, Francesco; Yang, Jenny J.
Afiliação
  • Reddish FN; Department of Chemistry, Center for Diagnostics and Therapeutics, Advanced Translational Imaging Facility, Georgia State University, Atlanta, GA 30303, USA.
  • Miller CL; Department of Chemistry, Center for Diagnostics and Therapeutics, Advanced Translational Imaging Facility, Georgia State University, Atlanta, GA 30303, USA.
  • Deng X; Department of Chemistry, Center for Diagnostics and Therapeutics, Advanced Translational Imaging Facility, Georgia State University, Atlanta, GA 30303, USA.
  • Dong B; Department of Chemistry, Center for Diagnostics and Therapeutics, Advanced Translational Imaging Facility, Georgia State University, Atlanta, GA 30303, USA.
  • Patel AA; Neuroscience Institute, Georgia State University, Atlanta, GA 30303, USA.
  • Ghane MA; Center for Behavioral Neuroscience, Georgia State University, Atlanta, GA 30303, USA.
  • Mosca B; Neuroscience Institute, Georgia State University, Atlanta, GA 30303, USA.
  • McBean C; Center for Behavioral Neuroscience, Georgia State University, Atlanta, GA 30303, USA.
  • Wu S; Department of Life Sciences, General Pathology, University of Ferrara, Ferrara, Italy.
  • Solntsev KM; Department of Chemistry, Center for Diagnostics and Therapeutics, Advanced Translational Imaging Facility, Georgia State University, Atlanta, GA 30303, USA.
  • Zhuo Y; Center for Molecular and Translational Medicine, Georgia State University, Atlanta, GA 30303, USA.
  • Gadda G; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Fang N; Department of Chemistry, Center for Diagnostics and Therapeutics, Advanced Translational Imaging Facility, Georgia State University, Atlanta, GA 30303, USA.
  • Cox DN; Department of Chemistry, Center for Diagnostics and Therapeutics, Advanced Translational Imaging Facility, Georgia State University, Atlanta, GA 30303, USA.
  • Mabb AM; Department of Chemistry, Center for Diagnostics and Therapeutics, Advanced Translational Imaging Facility, Georgia State University, Atlanta, GA 30303, USA.
  • Treves S; Neuroscience Institute, Georgia State University, Atlanta, GA 30303, USA.
  • Zorzato F; Center for Behavioral Neuroscience, Georgia State University, Atlanta, GA 30303, USA.
  • Yang JJ; Neuroscience Institute, Georgia State University, Atlanta, GA 30303, USA.
iScience ; 24(3): 102129, 2021 Mar 19.
Article em En | MEDLINE | ID: mdl-33665552
The precise spatiotemporal characteristics of subcellular calcium (Ca2+) transients are critical for the physiological processes. Here we report a green Ca2+ sensor called "G-CatchER+" using a protein design to report rapid local ER Ca2+ dynamics with significantly improved folding properties. G-CatchER+ exhibits a superior Ca2+ on rate to G-CEPIA1er and has a Ca2+-induced fluorescence lifetimes increase. G-CatchER+ also reports agonist/antagonist triggered Ca2+ dynamics in several cell types including primary neurons that are orchestrated by IP3Rs, RyRs, and SERCAs with an ability to differentiate expression. Upon localization to the lumen of the RyR channel (G-CatchER+-JP45), we report a rapid local Ca2+ release that is likely due to calsequestrin. Transgenic expression of G-CatchER+ in Drosophila muscle demonstrates its utility as an in vivo reporter of stimulus-evoked SR local Ca2+ dynamics. G-CatchER+ will be an invaluable tool to examine local ER/SR Ca2+ dynamics and facilitate drug development associated with ER dysfunction.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: IScience Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: IScience Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos